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KMID : 1005220140290010051
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Journal of Embryo Transfer
2014 Volume.29 No. 1 p.51 ~ p.57
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In Vitro Development of Porcine Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa with Trehalose
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Kang Hwa-Hyung
Lee Ji-Woong
Kang Man-Jong
Kim Kwang-Hyun
Moon Seung-Ju
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Abstract
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The objectives of this study were to investigate the effects of trehalose as a cryoprotectant for porcine freeze-dried spermatozoa, to find the optimal freeze-drying time and storage periods of freeze-dried spermatozoa, and to find out pronuclear formation rates, cleaved rates, and embryo development through intracytoplasmic injection of freeze-dried spermatozoa on porcine oocytes. The survival rates of spermatozoa after freeze-drying with trehalose treatment were significantly higher than those of them without trehalose treatment (p<0.05). The highest survival rates were found at 75 mM trehalose treatment. The longer storage periods after freeze-drying seemed to have a lower survival rates. Development in culture of pig by ICSI with trehalose treatment were significantly higher than those of them without trehalose treatment (p<0.05). Shorter freeze-drying time of spermatozoa was resulted in the highest cleaved rates and embryo development.
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KEYWORD
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trehalose , porcine , freeze-dried spermatozoa , survival rates of spermatozoa , cleaved rates , and embryo development
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